Restriction enzymes
- A
Unwind double-stranded DNA at specific origins, exposing single strands for replication
- B
Join DNA fragments at compatible ends using ATP-dependent ligase enzyme activity
- Ccheck_circle
Cut DNA at specific recognition sequences (often palindromic), producing fragments with sticky ends
- D
Synthesize DNA from RNA templates using reverse transcriptase activity in vitro
Explanation
Bacterial enzymes used in cloning. Sticky-ended fragments can be ligated to other DNA cut with the same enzyme.