Quantitative PCR (qPCR) was performed on cDNA from three tissues to compare gene expression of GeneX. Cycle threshold (Ct) values are reported (lower Ct = more starting transcript).
Which tissue has the highest GeneX expression?
- Acheck_circle
Liver, because lower Ct means more starting transcript.
- B
All three express GeneX equally.
- C
Muscle, because higher Ct means more transcript.
- D
Brain, because Ct is intermediate.
Explanation
qPCR Ct is the cycle at which fluorescence crosses threshold; samples with more starting template reach threshold sooner (lower Ct). Liver (Ct=18) has the most transcript.