Cell counts on each plate (approximate colonies):
<ul> <li>P1 LB only: lawn (uncountable)</li> <li>P2 LB+amp: 240 colonies, none fluoresce</li> <li>P3 LB+amp+ara: 235 colonies, all fluoresce</li> <li>P4 LB+ara: lawn, none fluoresce visibly</li> </ul>What is the calculated transformation efficiency (transformants per microgram DNA) if 0.5 microgram of plasmid was used?
- A
About 120 transformants/microgram
- B
Cannot be calculated without dilution data
- Ccheck_circle
About 480 transformants/microgram
- D
About 240 transformants/microgram
Explanation
Transformants per microgram = colonies on selective plate / micrograms DNA. Using P2: 240 / 0.5 = 480 transformants per microgram. (In real experiments dilution and plating volume corrections may also apply.)