Biotechnology

AP Biology· difficulty 3/5

Polymerase chain reaction (PCR) amplifies specific DNA sequences by cycling through

  • A

    Transcription (mRNA synthesis), processing (capping and splicing), translation (protein synthesis)

  • B

    Restriction (enzyme cutting), ligation (joining fragments), transformation (uptake by bacteria)

  • C

    Denaturation (heat), annealing (primers bind), extension (Taq polymerase synthesizes)

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  • D

    Initiation (origin recognition), elongation (DNA synthesis), termination (telomere capping)

Explanation

Each cycle doubles the target DNA. Taq polymerase from thermophilic bacteria survives the high-temperature denaturation step.

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