Which feature of Sanger sequencing produces individual peaks corresponding to each base?
- Acheck_circle
Incorporation of fluorescently labeled, chain-terminating ddNTPs that stop synthesis at random positions.
- B
PCR with mismatched primers.
- C
Reverse transcription of an mRNA template.
- D
Use of restriction enzymes that cut after each nucleotide.
Explanation
Each ddNTP terminates DNA synthesis at the corresponding base. After capillary electrophoresis, the dye color identifies which base terminated each fragment, producing the chromatogram peaks.