What is the most important advantage of using cDNA rather than genomic DNA for cloning a eukaryotic gene into a bacterial expression vector?
- A
cDNA encodes only ribosomal RNA.
- B
cDNA contains regulatory enhancers absent from genomic DNA.
- Ccheck_circle
cDNA lacks introns, which bacteria cannot splice out.
- D
cDNA has a longer half-life than genomic DNA.
Explanation
Bacteria lack spliceosomes; cloning intron-free cDNA (from mature mRNA) ensures the bacterial machinery can express the eukaryotic protein correctly.