Biotechnology

AP Biology· difficulty 3/5

CRISPR-Cas9 is a gene-editing system that

  • A

    Uses a complementary RNA template to direct reverse transcriptase to a specific gene, where it copies the sequence

  • B

    Uses a recognition sequence to direct a restriction enzyme to a specific palindrome, where it cuts the DNA

  • C

    Uses a guide protein to direct an RNA polymerase to a specific promoter, where it activates transcription

  • D

    Uses a guide RNA to direct the Cas9 nuclease to a specific DNA sequence, where it makes a cut for editing

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Explanation

Adapted from bacterial defenses against viruses, CRISPR enables targeted, precise editing of any sequence specified by the guide RNA.

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