Biotechnology

AP Biology· difficulty 2/5

A DNA sample run alongside a size ladder produces two visible bands.

- (cathode, top) + (anode, bottom) 10 kb 5 kb 1 kb 0.2 kb sample

Approximately what fragment sizes does the sample contain?

  • A

    Both bands are below 0.2 kb

  • B

    About 6 kb and about 0.5 kb

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  • C

    Cannot estimate without sequence data

  • D

    Both bands are 10 kb

Explanation

DNA migrates toward the positive electrode; smaller fragments travel farther. Comparing to the ladder, the sample bands fall between 5-10 kb (<del>6 kb) and between 1-0.2 kb (</del>0.5 kb).

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