Beer-Lambert Law

AP Chemistry· difficulty 2/5

A student prepares five aqueous CuSO4 standard solutions by dilution from a 0.500 M stock and measures their absorbance at 635 nm in a 1.00 cm cuvette. The blank is distilled water. Concentrations and absorbances are: 0.050 M (A=0.105), 0.100 M (A=0.210), 0.200 M (A=0.420), 0.300 M (A=0.630), 0.400 M (A=0.840). An unknown CuSO4 solution gives A=0.525 in the same cuvette.

Absorbance [CuSO4] (M) A = 2.10 [CuSO4]

What is the concentration of the unknown CuSO4 solution?

  • A

    0.105 M

  • B

    1.10 M

  • C

    0.525 M

  • D

    0.250 M

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Explanation

The slope is 2.10 M^-1 (e.g., 0.840/0.400). Using A = slope * c, c = 0.525 / 2.10 = 0.250 M.

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