Membrane Transport

AP Biology· difficulty 3/5

A student investigates the surface-area-to-volume effect on diffusion. She prepares agar cubes (containing the pH indicator phenolphthalein, pink in base) of three sizes: 1 cm, 2 cm, and 3 cm on a side. Cubes are placed in 0.1 M NaOH and timed for the pink color to fade completely (indicating NaOH has diffused throughout the cube and neutralized the indicator). The starting color is uniform pink in all cubes.

1 cm 2 cm 3 cm Phenolphthalein cubes after 5 min in NaOH violet = unreacted region

Which cube is predicted to lose its pink color first?

  • A

    The 1-cm cube

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  • B

    The 2-cm cube

  • C

    The 3-cm cube

  • D

    All three at the same time

Explanation

Surface-to-volume ratio decreases with cube size. The 1-cm cube has the highest SA:V (6:1), so NaOH penetrates the entire volume fastest. Larger cubes have proportionally less surface for diffusion per unit volume.

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