What does the absence of a band in the KO lane and its return in the Rescue lane indicate?
- Acheck_circle
Gene X is required for production of protein X, and reintroducing it restores expression.
- B
The antibody is non-specific.
- C
Protein X is processed into smaller fragments only in the KO.
- D
Protein X is produced post-mortem.
Explanation
Western blotting detects proteins via antibodies. Loss of the band in the knockout and its restoration upon gene re-expression demonstrates that gene X is necessary and sufficient for producing protein X.