A third trial added a putative inhibitor at fixed concentration to catalase. Reaction rate was measured across the same range of [H2O2]. Vmax of the uninhibited enzyme (4.1 mL/s) was unchanged, but more substrate was needed to approach Vmax.
The data are most consistent with which type of inhibition?
- A
Noncompetitive inhibition
- B
Irreversible denaturation
- C
Allosteric activation
- Dcheck_circle
Competitive inhibition
Explanation
Competitive inhibitors raise the apparent Km (more substrate needed) but Vmax can still be reached at high [S]. Noncompetitive inhibitors lower Vmax.