Enzyme Catalysis

AP Biology· difficulty 3/5

Researchers added inhibitor X to an enzyme assay. Both curves approach the same Vmax at high substrate concentrations, but the inhibited curve has a higher Km.

[Substrate] Rate No inhibitor + Inhibitor X

What type of inhibition is most consistent with these observations?

  • A

    Irreversible covalent inhibition

  • B

    Noncompetitive inhibition

  • C

    Competitive inhibition

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  • D

    Allosteric activation

Explanation

Competitive inhibitors bind the active site, raising the apparent Km but not changing Vmax because excess substrate can outcompete the inhibitor.

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