Enzyme Catalysis

AP Biology· difficulty 3/5

A second team measured catalase activity at 10 mM H2O2 across a pH range from 4 to 10. Reactions ran for 30 seconds at 25 degrees C. Each bar shows mean rate (mL O2/s).

pH Rate 4 5 6 7 8 10

What does the pattern suggest about catalase?

  • A

    It functions equally well across all tested pH values.

  • B

    Activity increases linearly with pH.

  • C

    It functions only in acidic environments.

  • D

    Its optimal pH is near 7, with sharply reduced activity at extreme pH.

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Explanation

Activity peaks at pH 7 and falls off on either side. Extreme pH disrupts the active-site shape and ionization of side chains, lowering catalytic efficiency.

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