Mutations

AP Biology· difficulty 3/5

The mRNA of beta-globin in sickle-cell hemoglobin substitutes valine (Val) for the wild-type glutamate (Glu) at codon 6.

WT mRNA: 5'... GAA GAG GUG CAU ... 3' ... Glu Glu Val His ... Mut mRNA: 5'... GAA GUG GUG CAU ... 3' ... Glu Val Val His ... Compare WT vs Mut codons

What single-nucleotide change accounts for this Glu-to-Val substitution?

  • A

    Loss of the start codon.

  • B

    Insertion of an extra base, causing a frameshift.

  • C

    Methylation of histone H3 at the locus.

  • D

    An A-to-T point substitution that changes GAG to GUG (in DNA: GAG to GTG).

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Explanation

GAG (Glu) becomes GUG (Val) by substituting the middle A with U (DNA template change A->T). This single missense mutation in the beta-globin gene causes sickle-cell disease.

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