Replication

AP Biology· difficulty 2/5

A replication fork is shown with a leading and lagging strand. Helicase unwinds DNA at the fork.

parental leading strand lagging strand Okazaki 5' to 3' helicase

Why is the lagging strand synthesized in short Okazaki fragments?

  • A

    The lagging strand uses a different polymerase that synthesizes 3' to 5'.

  • B

    DNA polymerase only adds nucleotides 5' to 3', so the strand opposite to fork movement must be made discontinuously.

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  • C

    RNA primers are not required on the lagging strand.

  • D

    Helicase repeatedly stops on the lagging strand template.

Explanation

DNA polymerase synthesizes only in the 5' to 3' direction. On the lagging-strand template, this requires repeated short syntheses (Okazaki fragments) that are later joined by ligase.

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