Introduction to Signal Transduction

AP Biology· difficulty 3/5

The figure shows nucleotide exchange on the alpha subunit of a heterotrimeric G protein during signal activation.

Inactive G protein (GDP bound) GDP Ligand binds receptor Active G protein (GTP bound) GTP α subunit dissociates and activates effector enzyme

Which event activates the alpha subunit of a G protein?

  • A

    Methylation of the receptor's third cytoplasmic loop

  • B

    Phosphorylation of GDP to ATP

  • C

    Exchange of GDP for GTP on the alpha subunit

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  • D

    Removal of the gamma subunit by proteolysis

Explanation

Ligand-bound GPCRs catalyze GDP-to-GTP exchange on the alpha subunit. GTP-bound alpha then dissociates and binds downstream effectors such as adenylyl cyclase.

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