Absorbance spectrum of an unknown shows a peak at the dashed wavelength.
For most accurate Beer-Lambert quantitation, measurements should be made at:
- Acheck_circle
lambda_max (the wavelength of maximum absorbance)
- B
Any wavelength
- C
The shortest wavelength shown
- D
The longest wavelength shown
Explanation
Working at lambda_max maximizes signal-to-noise and minimizes errors from small wavelength uncertainties because the curve is locally flat at the peak.