Cellular Respiration

AP Biology· difficulty 3/5

The same germinating-seed respirometer was used at 25 deg C, but seeds were poisoned with KCN (a cytochrome c oxidase inhibitor) in one trial and untreated in the other.

Time (min) O2 used Untreated + KCN

Why does KCN nearly eliminate O2 consumption?

  • A

    KCN inhibits glycolysis.

  • B

    Cytochrome c oxidase passes electrons to O2; blocking it halts the entire ETC.

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  • C

    KCN dissolves the mitochondrial outer membrane.

  • D

    KCN replaces O2 as the terminal electron acceptor.

Explanation

Complex IV (cytochrome c oxidase) is the only enzyme that reduces O2 to water in the ETC. Blocking it stops all electron flow and O2 consumption.

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