Synchronized human cells were grown and DNA content per cell was measured by flow cytometry. The histogram shows the distribution of cells by DNA content (1n vs 2n).
Cells in the central plateau are most likely in which phase?
- A
G1 phase
- B
G2 phase
- C
Mitosis
- Dcheck_circle
S phase (DNA replication in progress)
Explanation
Cells with intermediate DNA content (between 2N and 4N) are actively replicating DNA, defining S phase. The two peaks correspond to G1 (2N) and G2/M (4N).