The standard steps in cloning a gene into a plasmid are
- A
Methylate DNA + plasmid with same enzyme; anneal; inject into bacteria; image
- B
Translate DNA into protein; ligate to plasmid; transcribe in vitro; sequence
- C
Sequence DNA + plasmid; mutate; transform into yeast cells; amplify by PCR
- Dcheck_circle
Cut DNA + plasmid with same restriction enzyme; ligate; transform into bacteria; select
Explanation
Cut both with the same enzyme to produce compatible sticky ends; ligate with DNA ligase; transform competent bacteria; select with antibiotics.