At the saturation point, why does adding more substrate fail to increase rate?
- A
Substrate becomes a competitive inhibitor at high concentration
- B
Substrate denatures the enzyme above saturation
- Ccheck_circle
All enzyme active sites are occupied; rate is limited by enzyme turnover
- D
Free energy reaches zero
Explanation
Above saturation, every enzyme is busy. Rate becomes limited by how fast enzymes can convert and release product (kcat), not by substrate availability.